Molecular pathogenesis of human amyloidosis: Lessons from β2-microglobulin-related amyloidosis
| Autor: | Daisaku Ozawa, Kazuhiro Hasegawa, Hironobu Naiki, Tadakazu Okoshi, Itaru Yamaguchi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Amyloid Beta-2 microglobulin Chemistry Endosome Amyloidosis Molecular pathogenesis macromolecular substances General Medicine Amyloid fibril medicine.disease In vitro Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry mental disorders medicine Thioflavin |
| Zdroj: | Pathology International. 66:193-201 |
| ISSN: | 1320-5463 |
| DOI: | 10.1111/pin.12394 |
| Popis: | Amyloidosis refers to a group of diseases with amyloid fibrils deposited in various organs and is classified into more than 30 diseases in humans based on the kind of amyloid protein. In order to elucidate the molecular pathogenesis of human amyloidosis, we studied the molecular mechanism of amyloid fibril formation in vitro. We first developed a novel fluorometric method to determine amyloid fibrils in vitro based on the unique characteristics of thioflavin T. We next proposed a nucleation-dependent polymerization model to explain the general mechanism of amyloid fibril formation in vitro. Based on this model, we characterized the biological molecular interactions that promote or inhibit amyloid fibril formation in vitro and developed models of pathological molecular environment for inducing human β2-microglobulin-related amyloidosis in long-term hemodialysis patients. We also proposed a novel and attractive cytotoxic mechanism of β2-microglobulin amyloid fibrils, that is, the disruption of endosomal/lysosomal membranes by endocytosed amyloid fibrils. These findings may be useful to elucidate the molecular pathogenesis of other kinds of human amyloidosis. |
| Databáze: | OpenAIRE |
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