Association of TGF-β2 Gene Polymorphism with Salmonella pullorum Bacterial Infection Resistance in Tolaki Chickens
| Autor: | Muh Akramullah, Niken Ulupi, Cece Sumantri, Muh. Amrullah Pagala |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | International Journal of Scientific Research in Science, Engineering and Technology. :46-54 |
| ISSN: | 2394-4099 2395-1990 |
| DOI: | 10.32628/ijsrset20716 |
| Popis: | Transformation growth factor-beta 2 gene (TGF-β2) is a gene which belongs to the cytokine gene group. The present study aimed to identify Single Nucleotide Polymorphism (SNP) g. 640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) and associate its genotypes with Salmonella pullorum Bacterial Infection Resistance in Tolaki Chickens. This study used blood samples collected from 70 chickens. Discussion was conducted on leucocyte concentration, leucocyte differentiation (heterophils, monocytes, lymphocytes, eosinophils, basophils and H/L ratio) and clearance test. Identification of Single Nucleotide Polymorphism of Transformation growth factor-beta 2 gene (TGF-β2) was performed by PCR-RFLP method. Association of (SNP) g. 640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) with leukocyte profiles and their differentiation and resistance to bacterial infections was carried out using analysis of variance with GLM (General Linear Model). SNP g.640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) was polymorphic and was in Hardy-Weinberg equilibrium. Based on molecular and biological testing, Tolaki chickens in genotypes (TT, TC and CC) have normal leucocyte concentration and differenciation and resistant to Salmonella pullorum. |
| Databáze: | OpenAIRE |
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