Application of chicken embryonated eggs as a new model for evaluating the virulence of Neospora caninum tachyzoites

Autor: M. Mansourian, Mohsen Lotfi, Monir Khordadmehr, Mohammad H. Hosseini, Abdollah Rahimiyan, Mehdi Namavari, Azizollah Khodakaram Tafti
Rok vydání: 2011
Předmět:
Zdroj: Comparative Clinical Pathology. 21:1665-1668
ISSN: 1618-565X
1618-5641
DOI: 10.1007/s00580-011-1346-9
Popis: Neospora caninum is a cyst-forming coccidian parasite, which is highly pathogenic for cows and dogs. Determination of parasite virulence has been applied in the development of live vaccines against protozoan parasites. In this sense, we wanted to determine whether chicken embryonated egg could be an animal model to show different virulence of N. caninum tachyzoites. In this study, the first N. caninum isolate was considered low passage (no passage) and then passaged for 80 times in vitro as high passage. Groups of 8-day-old embryonated eggs with 10 eggs in each group were inoculated with 10 and 102 of low or high-passage N. caninum tachyzoites, and any mortality was recorded. Suitable samples from different tissues (liver, heart, and brain) of the dead embryos were collected for histopathological and immunohistochemistry (IHC) study. In this study, the mortality rate decreased in the high passage infection in each group and IHC method showed the presence of the parasite in chicken tissues receiving high and low passage parasites. The present investigation showed that the chicken embryonated eggs can be a valuable alternative approach for in vivo testing of live attenuated potential vaccine, and also, these results confirm that an increase in passage could decrease the N. caninum virulence in chicken embryonated egg.
Databáze: OpenAIRE
Externí odkaz: