| Autor: |
R. Arnold, E. Hofer |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
IFMBE Proceedings ISBN: 9783642293047 |
| Popis: |
Intracellular measurement of action potential duration (APD) restitution is challenging in beating heart tissue preparations because of the risk of loosing impalement of glass microelectrodes during pacing. Therefore short-time pacing protocols are beneficial. In this work we show results from two short lasting pacing protocols applied on a guinea pig papillary muscle which could enhance APD restitution measurements in beating tissue preparations. Furthermore measurements at a large number of recording sites could be promoted by minimizing experiment duration and therefore prevent superfused tissue from suffering ischemia. Simultaneously measured extracellular parameters show large beat-to-beat variations prior to conduction block in one type of protocol (ramp-like). This could provide a basis for conduction block forecasting. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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