Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor
| Autor: | Zsofia Kutil, Zhu Li Yun, Jan Rezek, Ji Dong Lou, Tomas Vanek, Premysl Landa, Petr Marsik, Jan Tauchen, Lenka Langhansova |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Ethanol biology Chemistry Leukotriene B4 010401 analytical chemistry Immunology Ethyl acetate biology.organism_classification 01 natural sciences 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Enzyme Biosynthesis Botany Food science Agronomy and Crop Science IC50 Fruit tree Myrica rubra Food Science |
| Zdroj: | Food and Agricultural Immunology. 28:343-353 |
| ISSN: | 1465-3443 0954-0105 |
| DOI: | 10.1080/09540105.2016.1272554 |
| Popis: | Myrica rubra Sieb. et Zucc. is a valuable fruit tree that is used in Chinese, Japanese and Taiwanese traditional medicine. We investigated the anti-inflammatory activity of M. rubra leaves extracted with four different solvents. Total phenolics were determined using the Folin–Ciocalteu method. Extracts were investigated for their inhibitory activity toward the pro-inflammatory enzymes cyclooxygenase-1 and -2 (COX-1, COX-2) and 5-lipoxygenase (5-LOX). The ethanol extract of M. rubra leaves demonstrated a strong inhibition of prostaglandin E2 (PGE2) biosynthesis catalyzed by both COX-1 (93.42%) and COX-2 (75.71%) and leukotriene B4 (LTB4) formation catalyzed by 5-LOX (82.72%). Further we identified selective COX-1 inhibition by the n-butanol and aqueous fractions of the ethanol extract (with an IC50 for COX-1 inhibition of 1.07 and 0.71 µg mL−1 , respectively) and dual 5-LOX/COX inhibition by the ethyl acetate fraction (with an IC50 of 3.29 for COX-1, 2.54 for COX-2 and 8.30 µg mL−1 for 5-LOX). |
| Databáze: | OpenAIRE |
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