Effect of phase of medium, growth regulators and nutrient supplementations on in vitro shoot-tip necrosis in pear

Autor:	J. S. Kanwar, Anirudh Thakur
Rok vydání:	2011
Předmět:	chemistry.chemical_classification PEAR Necrosis Horticulture Biology In vitro nervous system diseases body regions surgical procedures operative Nutrient nervous system chemistry Auxin Botany Shoot Genotype medicine medicine.symptom Agronomy and Crop Science
Zdroj:	New Zealand Journal of Crop and Horticultural Science. 39:131-140
ISSN:	1175-8783 0114-0671
DOI:	10.1080/01140671.2011.559254
Popis:	The incidence of shoot-tip necrosis (STN) was observed during the in vitro shoot multiplication and rooting stages of five Pyrus genotypes. During shoot multiplication, there was no incidence of STN in any of the five pear genotypes after 2 weeks of culture. However, after 6 weeks of culturing, necrotic apices were observed in all the pear genotypes, except wild pear. After 8 weeks of culture, 79.31% STN was observed in Punjab Beauty, followed by Patharnakh (49.60%), kainth (24.65%), shiara (17.68%) and wild pear (7.32%). Irrespective of the genotype, STN increased with an increase in BA concentration only in 8-week-old cultures. During in vitro rooting, the incidence of STN also varied with the genotype. In all five pear genotypes, the use of liquid media for in vitro rhizogenesis was useful in reducing the incidence of necrotic apices. The auxin type and level also influenced the occurrence of STN significantly. In wild pear, the incidence of necrotic apices increased with an increase in the co...
Databáze:	OpenAIRE
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