Rapid Identification of Thermotolerant Campylobacter jejuni , Campylobacter coli , Campylobacter lari , and Campylobacter upsaliensis from Various Geographic Locations by a GTPase-Based PCR-Reverse Hybridization Assay
| Autor: | B. A. J. Giesendorf, Anita Verschuuren‐ van Haperen, Michael B. Huysmans, Wim Quint, Martin J. Blaser, Leen-Jan van Doorn, Peter Vandamme, André P. Burnens |
|---|---|
| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Journal of Clinical Microbiology. 37:1790-1796 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.37.6.1790-1796.1999 |
| Popis: | Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow amplification of a 153-bp fragment from C. jejuni , C. coli , C. lari , and C. upsaliensis . Sequence analysis of these PCR products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant Campylobacter species. Multiple probes were used to develop a line probe assay (LiPA) that permits analysis of PCR products by a single reverse hybridization step. A total of 320 reference strains and clinical isolates from various geographic origins were tested by the GTP-based PCR-LiPA. The PCR-LiPA is highly specific in comparison with conventional identification methods, including biochemical and whole-cell protein analyses. In conclusion, a simple method has been developed for rapid and highly specific identification of thermotolerant Campylobacter species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|