Une méthode d'immobilisation d'anticorps par liaisons covalentes sur nylon 66. Application en test immunoenzymatique

Autor:	Bernard Perrin, Guy Favre-Bonvin, Albert Ville, Roger Couturier
Rok vydání:	1987
Předmět:	chemistry.chemical_classification Nylon 66 Chemistry Lysine Phenylalanine medicine.disease_cause Amino acid Amidine chemistry.chemical_compound Covalent bond Amide Polymer chemistry medicine Escherichia coli
Zdroj:	Die Makromolekulare Chemie. 188:1305-1312
ISSN:	0025-116X
DOI:	10.1002/macp.1987.021880608
Popis:	A new method of immobilization of low-molecular-weight compounds or antibodies on nylon 66 was developed. Secondary amide groups of nylon 66 were converted into secondary imidate groups by O-alkylation with sulfuric acid and methanol. The methoxycarbonimidoyl groups react with amino compounds to produce stable amidine links. Antibodies and several low-molecular-weight compounds (amino acids) were immobilized by covalent linkage on nylon balls. Fixed antibodies were used in solid phase enzyme linked immunoassay, i.e. applied for the detection of K99+ Escherichia Coli in calves.
Databáze:	OpenAIRE
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