Transgenic Expression of a Bacterial Thermophilic Amylase in the Chlamydomonas reinhardtii Chloroplast to Facilitate Algal Biofuel Production
| Autor: | Yan Liu, Xiaoqing Wang, Zhenhua Ruan, Danielle Boileau, Wei Liao, Barbara B. Sears |
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| Rok vydání: | 2014 |
| Předmět: |
Renewable Energy
Sustainability and the Environment Starch Thermophile fungi food and beverages Chlamydomonas reinhardtii Biology biology.organism_classification Microbiology Chloroplast chemistry.chemical_compound chemistry Biochemistry Enzymatic hydrolysis biology.protein Amylase Alpha-amylase Agronomy and Crop Science Thermotoga neapolitana Energy (miscellaneous) |
| Zdroj: | BioEnergy Research. 8:527-536 |
| ISSN: | 1939-1242 1939-1234 |
| Popis: | This study investigated expression of a thermophilic amylase (arAmyBH) from hyperthermophilic bacterium Thermotoga neapolitana into Chlamydomonas reinhardtii chloroplast to facilitate in vivo starch hydrolysis for algal biofuel production. The expression and accumulation of the recombinant protein in the algal chloroplast were verified by Western blot analysis. The recombinant arAmyBH was purified using Ni-NTA purification system. The optimum pH and temperature of arAmyBH were 5.5 and 60 °C, respectively. The enzymatic hydrolysis of the algal starch demonstrated that with supplemental commercial alpha-amylase, the transformant completely converted starch into reducing sugars in vivo at an elevated temperature. |
| Databáze: | OpenAIRE |
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