Versatile Method for Staining Insect Eggs and Larvae in Leaves

Autor:	G. C. Tewari, Krishna P. N. Moorthy, M. P. Alexander
Rok vydání:	1988
Předmět:	Empoasca Ecology fungi food and beverages General Medicine Biology biology.organism_classification Acid fuchsin Staining Lactic acid chemistry.chemical_compound Acetic acid chemistry Agromyzidae Insect Science Botany Malachite green Fixative
Zdroj:	Journal of Economic Entomology. 81:403-405
ISSN:	1938-291X 0022-0493
DOI:	10.1093/jee/81.1.403
Popis:	A technique to stain insect eggs and larvae within leaf tissue is described. The leaves are first treated with modified Carnoy’s fixative (ethyl alcohol, chloroform, and glacial acetic acid in 6:4:3 proportion by volume) to remove chlorophyll, and then treated with clearing-staining solution (1:10 solution of 1% aqueous acid fuchsin in a clearing medium [20 g phenol and 5 g chloral hydrate in 100 ml lactic acid]) to stain eggs. Counterstaining is done with 0.1% malachite green in lactic acid. The eggs are stained crimson and plant tissues green. The method is effective in staining eggs of agromyzid flies, Ophiomyia phaseoli (Tryon) and Ophiomyia sp., and cicadellids, Empoasca kerii Pruthi and Amrasca biguttula biguttula Ishida, in their respective host plants. Larvae of O. phaseoli are stained as well. A modification of the procedure allows unstained eggs of O. phaseoli to be observed within ovipositional punctures; leaves are transferred directly from fixative to malachite green and observed under the microscope.
Databáze:	OpenAIRE
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