Quantitative determination of the total content γ-pyrone Compounds in the aerial part of Halenia corniculata by HPLC
| Autor: | Tatiana M. Shishmareva |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Butlerov Communications. 64:116-120 |
| ISSN: | 2074-0948 2074-0212 |
| DOI: | 10.37952/roi-jbc-01/20-64-11-116 |
| Popis: | This work presents the results of a quantitative determination of the total content of γ-pyrone compounds in the aerial part of Halenia corniculata using the HPLC method, where the standard samples of 1-hydroxy-2,3,5-trimethoxyxanthone and 1-hydroxy-2,3,4,5-tetramethoxyxanthone. The main active ingredients of H. corniculata are xanthones and flavones, and this plant can be used as a possible source of available phenol compounds. To determine the total content of xanthones and flavones in the aerial part of H. corniculata, the hexane, chloroform, and ethyl acetate fractions were studied. During the experiment, 13 compounds were found in the hexane fraction of H. corniculata, among which the content of 1-hydroxy-2,3,4,5-tetramethoxyxanthone is 43.07% of the mass of the hexane fraction and 1.44% of the mass of absolutely dry raw materials, and 1-hydroxy-2,3,5-trimethoxyxanthone – 22.20% by weight of the hexane fraction and 0.74% by weight of absolutely dry raw materials. In the chloroform fraction of H. corniculata, 17 compounds were found, and 1-hydroxy-2,3,4,5-tetramethoxyxanthone (6.26% of the mass of the chloroform fraction and 0.20% of the mass of absolutely dry raw materials) and 1-hydroxy-2,3,5-trimethoxyxanthone (4.22% by weight of the chloroform fraction and 0.13% by weight of absolutely dry raw materials). In the study of the ethyl acetate fraction of H. corniculata, 14 compounds were found, among which 1 component was identified – luteolin, the content of which is 68.26% of the weight of the ethyl acetate fraction and 2.41% of the weight of absolutely dry raw material. The total content of γ-pyrone compounds in the aerial part of H. corniculata is 4.92% (based on the weight of the absolute dry raw material). |
| Databáze: | OpenAIRE |
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