High-speed, multi-Z confocal microscopy for voltage imaging in densely labeled neuronal populations
| Autor: | Timothy D. Weber, Maria V. Moya, Jerome Mertz, Michael N. Economo |
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| Rok vydání: | 2021 |
| DOI: | 10.1101/2021.12.10.472140 |
| Popis: | Genetically encoded voltage indicators (GEVIs) hold great promise for monitoring neuronal population activity, but GEVI imaging in dense neuronal populations remains difficult due to a lack of contrast and/or speed. To address this challenge, we developed a novel confocal microscope that allows simultaneous multiplane imaging with high-contrast at near-kHz rates. This approach enables high signal-to-noise ratio voltage imaging in densely labeled populations and minimizes optical crosstalk during concurrent optogenetic photostimulation. |
| Databáze: | OpenAIRE |
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