Microbiological assay with Bacillus licheniformis for the easy detection of quinolones in milk
| Autor: | O. G. Nagel, Maria Pilar Molina, Melisa Tumini, Rafael Althaus |
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| Rok vydání: | 2017 |
| Předmět: |
Chromatography
medicine.drug_class Danofloxacin 010401 analytical chemistry Microbiological assay Antibiotics 0402 animal and dairy science 04 agricultural and veterinary sciences Biology biology.organism_classification 040201 dairy & animal science 01 natural sciences Applied Microbiology and Biotechnology 0104 chemical sciences Ciprofloxacin Sarafloxacin Marbofloxacin Enrofloxacin medicine Bacillus licheniformis Food Science medicine.drug |
| Zdroj: | International Dairy Journal. 64:9-13 |
| ISSN: | 0958-6946 |
| DOI: | 10.1016/j.idairyj.2016.08.008 |
| Popis: | This paper proposes a microbiological method in microtitre plates for the detection of residues of quinolones in milk. The method uses spores of Bacillus licheniformis in culture medium with a redox combination of indicators and gives a response time of 5.5 h. This method detects 92 μg L−1 of ciprofloxacin, 63 μg L−1 of danofloxacin, 109 μg L−1 of enrofloxacin, 101 μg L−1 of marbofloxacin and 109 μg L−1 of sarafloxacin in milk. Therefore, the assay is easy to develop and to use in laboratory, allowing analysis of large numbers of samples at low cost. Due to its good sensitivity to quinolones, this assay can be used as a complementary test of commercial microbiological methods and thereby improve food security. |
| Databáze: | OpenAIRE |
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