Construction and characterization of a bacterial artificial chromosome library of marine macroalgaPorphyra yezoensis (Rhodophyta)

Autor:	Jin Song Xuan, Ji Liang Yang, Bin Wang, Man Li Weng, Ge Zhao, Lin De Duan, Daiyong Deng
Rok vydání:	2004
Předmět:	Genetics Bacterial artificial chromosome biology Plant Science Porphyra yezoensis Molecular cloning biology.organism_classification Molecular Biology Genome Gene Insert (molecular biology) Porphyra Nuclear DNA
Zdroj:	Plant Molecular Biology Reporter. 22:375-386
ISSN:	1572-9818 0735-9640
DOI:	10.1007/bf02772680
Popis:	A large-DNA-fragment library is necessary for research into the Porphyra genome. In this study, a bacterial artificial chromosome (BAC) library of Porphyra yezoensis was constructed and characterized. The library contains 54,144 BAC clones with an average insert size of about 65 kb and fewer than 0.7% of clones without large inserts. Therefore, its capacity is more than 6.6 P. yezoensis genome equivalents, and the probability of recovering any nuclear DNA sequence from the library is higher than 99%. The library shows good fidelity and stability. A putative trehalose-6-phosphate synthase (TPS) gene was successfully screened out from the library. The above results show that the library is useful for gene cloning and genomic research in P. yezoensis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3e7c0a264b9dea13daa3e3753463e30c https://doi.org/10.1007/bf02772680 Zobrazit plný text záznamu Plný text ve formátu PDF