Amelotin-Mediated Crystals Assembly in Liquid-Phase and on Tooth Surface

Autor:	Xiaohua Ren, Min Zhou, Yandong Mu, Kun Tian
Rok vydání:	2021
Předmět:	Perpendicular direction Materials science Enamel paint visual_art Developing tooth Biophysics visual_art.visual_art_medium Tooth surface Liquid phase Transmission electronic microscopy Electronic microscopy Enamel Formation
Zdroj:	Journal of Materials Science and Chemical Engineering. :35-44
ISSN:	2327-6053 2327-6045
Popis:	Objectives: To study the role of amelotin in enamel formation, purified recombinant human amelotin was used in a study of amelotin self assembly and its role in new HAP crstal structure formation in a laboratory setting. Methods: The full-length human amelotin gene was cloned from developing tooth buds and recombinant amelotin was expressed and purified. Morphological changes during amelotin self-assembly and the role of amelotin in promoting new minieralization structure is studied using Transmission electronic microscopy and Scanning electron microscopy. Results: The measured molecular weight of expressed human amelotin was 28 kDa that matches the predicted values. Amelotin self-assembles to form nano-spheres 10 - 30 nm in size. The amelotin self-assembly process further produces higher 3-D structure in the forms of chain-like fabric arranged in parallel and perpendicular direction. The neonatal pearl crystals were arranged side by side to form a string of small rods and small parallel rods extend from the demineralized glaze on the surface of teeth that were pre-treated with amelotin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3e6b0edcf1bcdac3c76e7d8c87bbcbac https://doi.org/10.4236/msce.2021.97004 Zobrazit plný text záznamu