| Autor: |
Byung Yun Cho, Dai Woon Lee |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Journal of Liquid Chromatography. 17:2541-2558 |
| ISSN: |
0148-3919 |
| DOI: |
10.1080/10826079408013396 |
| Popis: |
The retention behavior of proteins was investigated in reversed phase chromatography (RPC) and hydrophobic interaction chromatography (HIC). The observation was performed in three viewpoints : (1) What are the differences in the retention behavior between small molecules and proteins? (2) What are the differences between RPC and HIC of proteins and the reason of the differences? (3) How can be detected the structural changes of proteins eluted from the column? The retention behavior of proteins was able to be understood by the parameters used in describing the retention behavior of small molecules. However, applicable range of the parameters for proteins was limited and, sometimes, peculier behavior appeard in RPC and HIC for proteins; that is, the retention time of proteins showed extreme difference for the small change of organic fraction and showed the skewed U-shaped dependence on fraction of organic solvent. Even though the retention mechanism of proteins in RPC and HIC was based on the same... |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
