Influence of Column Material on Efficiency in High Speed Liquid Chromatography
Autor: | Howard Barth, Barry L. Karger |
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Rok vydání: | 1971 |
Předmět: |
Heptane
Chromatography Steady state Biochemistry (medical) Clinical Biochemistry Analytical chemistry chemistry.chemical_element Biochemistry Copper Column (database) Analytical Chemistry chemistry.chemical_compound Countercurrent chromatography Column chromatography chemistry Aluminium Electrochemistry Chromatography column Spectroscopy |
Zdroj: | Analytical Letters. 4:595-602 |
ISSN: | 1532-236X 0003-2719 |
DOI: | 10.1080/00032717108058656 |
Popis: | The influence of column tubular material on column efficiency in high speed liquid chromatography has been examined. For good reproducibility in HETP, the columns were first packed dry with Corasil I (28–37μ) and then heptane saturated with 3,3′-oxydipropionitrile was flown through the column. When steady state conditions were achieved, the % loading of stationary phase was 1.1%. It was found that column materials of precision bore and seamless stainless steel, aluminum, and copper gave equivalent efficiency results for retained and unretained components. Teflon coated aluminum columns were not reproducible, in regard to HETP. |
Databáze: | OpenAIRE |
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