Induction and cultivation of cloned filaments of Polysiphonia urceolata (Rhodomelaceae, Rhodophyta)

Autor: Kuishuang Shao, Bin Cheng, Qinqin Lu, Jin-Xia Wang, Bai-Cheng Zhou
Rok vydání: 2011
Předmět:
Zdroj: Chinese Journal of Oceanology and Limnology. 29:1260-1266
ISSN: 1993-5005
0254-4059
DOI: 10.1007/s00343-011-0295-0
Popis: A filamentous clone of Polysiphonia urceolata was regenerated from segments cut from the fronds of gametophytes. Unlike wild thalli with short virgate branchlets, the clone was filamentous with few branches. Many transparent trichoblasts arose from pericentral cells during the induction culture, but these were seldom observed during normal growth. The trichoblasts were uniseriate, often colorless, and formed lobed rhizoids rapidly when they came into contact with solid substrates. In addition to morphological characteristics, the photosynthetic properties and growth conditions of the clone differed from those of the mother plant. Cross-gradient light and temperature culture experiments revealed that the most favorable conditions for culture of the filamentous clone were 22 degrees C and 95-120 mu E/(m(2).s) light intensity. The photosynthetic light saturation value for filaments was approx. 100 mu E/(m(2).s), which is far lower than that of wild thalli. These results could be used to develop techniques for mass cultures of P. urceolata in photobioreactors for production of seed stock or bioactive products.
Databáze: OpenAIRE
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