POTENTIAL ROLE OF ANNONA SQUAMOSA LINN EXTRACT AND N-ACETYL CYSTEINE IN MANAGEMENT OF RIFAMPICIN-INDUCED HEPATOXICITY IN RATS

Autor:	Fatma Mokhtar Fouda, Hader Fawzy Darwish, Samiha Mohamed Abd Eldayem, Bosy Azmy Abd El Motelp
Rok vydání:	2020
Předmět:	Pharmacology Antioxidant biology Chemistry Bilirubin medicine.medical_treatment Glutathione reductase Albumin Pharmaceutical Science Glutathione Annona squamosa biology.organism_classification chemistry.chemical_compound Apoptosis Oral administration medicine
Zdroj:	International Journal of Pharmacy and Pharmaceutical Sciences. :117-123
ISSN:	0975-1491 2656-0097
DOI:	10.22159/ijpps.2020v12i8.38424
Popis:	Objective: Rifampicin (RIF) could be a recognized therapeutic and preventive agent against tuberculosis. Still, high rates of many side effects and symptoms related to hepatotoxicity have identified during treatment. So, the current study was aimed to evaluate the antioxidant and hepatoprotective activity of methanolic extract of Annona Squamosa Linn (MEAS) and N-Acetyl Cysteine (NAC) against RIF induced hepatic injury in male rats. Methods: The hepatoprotective effects of MEAS (500 mg/kg b.wt.) and NAC (100 mg/kg b.wt.) or co-treatment were assessed in a model of hepatotoxicity by RIF (300 mg/kg b. wt.) in male rats daily for 21 d. Moreover, bilirubin, total protein, albumin, ALT, AST, ALP, GGT, MDA, and GSH were estimated. In addition, the levels of IL-6, IL-10, 8(OH)dG, and Bcl2 were evaluated. Results: The oral administration of MEAS and NAC or their combination resulted in significant reductions in the levels of bilirubin, albumin, hepato-specific markers namely ALT, AST, ALP, GGT, and MDA as compared to the RIF group. Furthermore, MEAS and NAC or the combination of MEAS and NAC treatment significantly up-regulated the levels of total protein, glutathione reductase with concomitant decrease in inflammatory marker level IL-6 and apoptotic marker level 8(OH)dG as well as increment the level of anti-inflammatory marker IL-10 and anti-apoptotic marker Bcl2 as compared to the RIF group. Histological examination of the liver tissue indicated that co-treatment with MEAS and NAC completely abolished the inflammation and degeneration in hepatocytes and restore the liver tissue to its normal structure. Conclusion: The present findings demonstrated that a co-treatment of MEAS and NAC seems to be more productive and curative than alone MEAS or NAC treatment and strongly compensated the liver damage induced by RIF.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3e1cdf346aa23c5a781d53ee6044828a https://doi.org/10.22159/ijpps.2020v12i8.38424 Zobrazit plný text záznamu