Response of bEnd.3 cells to growing behavior on the graphene oxide film with 2-D grating structure by two-beam laser interference
Autor: | Zhankun Weng, Jin Yan, Li Li, Zuobin Wang, Chengcheng Xie, Yan Liu, Hongmei Xu, Zhengxun Song, Lu Wang, Liang Cao |
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Rok vydání: | 2021 |
Předmět: |
Materials science
Materials Science (miscellaneous) Oxide Nanochemistry Nanotechnology 02 engineering and technology Substrate (electronics) Grating 010402 general chemistry 01 natural sciences Nanomaterials law.invention chemistry.chemical_compound Tissue engineering law Electrical and Electronic Engineering Physical and Theoretical Chemistry Graphene Cell Biology 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences chemistry 0210 nano-technology Biosensor Biotechnology |
Zdroj: | Applied Nanoscience. 11:1141-1149 |
ISSN: | 2190-5517 2190-5509 |
Popis: | Graphene (G) and its derivatives are important nanomaterials with potential medical applications for biosensors and implanting biomaterials. The hydrophobicity and surface microstructures of substrates have great influences on the biological and physical properties of the surface-bound cells. In this work, we used the two-beam laser interference (TBLI) technique to prepare a two-dimensional (2-D) grating structure on the surface of graphene oxide (GO) film. We investigated the effect of GO and the GO film with the 2-D grating structure substrates on the growth behavior of rat brain microvascular endothelial (bEnd.3) cells. The results demonstrated that the cell spreading area and the number of surface-bound cells were closely related to the hydrophobicity of the substrate and the presence of oxygen-containing functional groups (OCGs). Due to the interaction of laser and GO, the GO in the interference area was transformed into reduced graphene oxide (RGO). The grating-structured GO film significantly affected the direction of cell spreading and morphology. It has a good application prospect as a scaffold in tissue engineering, and promising applications in the fields that require highly directional growth of cells, such as nerve injury repair, tendon repair and regeneration. |
Databáze: | OpenAIRE |
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