Characterization of PolyA and PolyC mismatches by Raman spectroscopy
| Autor: | Yu-bo Liao, Yaoyong Meng, Ying Wang, Hao-dong Lei |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Materials science
Base pair Hydrogen bond business.industry Stacking Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Characterization (materials science) chemistry.chemical_compound symbols.namesake Crystallography Nuclear magnetic resonance Optics chemistry symbols Electrical and Electronic Engineering Raman spectroscopy business Conformational isomerism Cytosine |
| Zdroj: | Chinese Optics Letters. 6:61-63 |
| ISSN: | 1671-7694 |
| Popis: | A·C mismatches are studied by Raman spectral characterization of PolyA, PolyC, and their equimolar complex in solution of 0.14 mol/L Na+, pH7.0. Experimental results show that A·C mismatches occur to be A/B (mainly A) conformers, and unlike Watson-Crick base pairing, this kind of mismatches is stabilized by only one hydrogen bond involving cytosine N4H2 and adenine N7. The formation of A·C complex makes the base stacking interactions much stronger, and conformation of the backbone more ordered, which leads to obvious Raman hypochromic effect with some shifts in corresponding bands. |
| Databáze: | OpenAIRE |
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