Microbial levan from Brachybacterium phenoliresistens : Characterization and enhancement of production
| Autor: | Zaid A. Thabit, Tarek A. A. Moussa, Safaa A. S. Al-Qaysi, Sanaa Basheer Kadhem |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Rhizosphere Sucrose Strain (chemistry) Bioengineering Biology 16S ribosomal RNA biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Microbiology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Gene bank chemistry 010608 biotechnology Casein Proton NMR Food science Bacteria |
| Zdroj: | Process Biochemistry. 57:9-15 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2017.03.008 |
| Popis: | Levan producing bacteria was isolated from rhizosphere soil. The molecular identification of this isolate was conducted using 16S rRNA, which resulted in a sequenced region of 1298 base pairs. The sequence alignment in the gene bank indicated that this isolate has a high percentage of similarity (99%) to the retrieved consensus sequence of Brachybacterium phenoliresistens strain phenol-A. The produced levan was characterized using TLC, FTIR, 1H NMR and 13C NMR spectroscopy techniques. The effects of nutritional and physical factors on this isolate’s levan production were investigated. The results demonstrated that the optimal sources for carbon and casein during levan production were sucrose and casein, yielding 7.88 g/land 8.12 g/l of levan, respectively. The highest levan yield (7.97 g/l) was obtained at a sucrose concentration of 300 g/l. At an initial pH of 7.8, this bacterium yielded their highest levan production of 7.88 g/l. The optimal incubation period was 72 h with a yield of 8.58 g/l, the optimal temperature was 30 °C and resulted in 7.87 g/l, and the highest levan production yield was obtained at 150 rpm and yielded 8.12 g/l. |
| Databáze: | OpenAIRE |
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