Determination of Vitamin K1 in Medical Foods by Liquid Chromatography with Postcolumn Reduction and Fluorometric Detection
| Autor: | George M Ware, Ronald R. Eitenmiller, Chase Gw, Austin R Long |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pharmacology
Detection limit Vitamin Sodium bicarbonate Chromatography Chemistry Coefficient of variation Extraction (chemistry) Fluorescence spectrometry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Environmental Chemistry Agronomy and Crop Science Quantitative analysis (chemistry) Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 83:957-962 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | A liquid chromatographic (LC) method is described for the determination of vitamin K1 in medical foods. The sample is enzymatically digested with lipase and α-amylase and extracted with 1% sodium bicarbonate solution–isopropanol (1 + 1). After C18 solid-phase extraction, vitamin K1 is separated by nonaqueous reversed-phase LC, converted to the hydroquinone by postcolumn zinc reduction, and quantitated by fluorescence detection. The limit of detection is 8 pg (3 σ), and the limit of quantitation is 27 pg (10 σ) on column. Linear response ranged from 0.1 to 1.0 ng vitamin K1 (r = 0.9999). The mean recovery (n = 38) for all spiking levels was 101.6 ± 2.85%. Analysis of Standard Reference Material 1846, Infant Formula, gave a mean value of 0.95 ± 0.088 mg vitamin K/kg (K or K1?)(n = 31) with a coefficient of variation of 9.26. |
| Databáze: | OpenAIRE |
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