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BACKGROUND:CoptotermesformosanusShirakiwasaccidentallyintroducedintotheUnitedStatesfromAsia.Theintroduction of the pest has brought significant economic consequences. During the past decade, Mississippi has become a significantly infested state, partly due to the proximity to coastal port cities such as New Orleans. This study was initiated to investigate the origin and infestation route of C.formosanusin southern Mississippi. RESULTS: Twenty-eight colonies (21 colonies from Mississippi, six from Louisiana, one from China) were collected. Sequencing and analysis of 112 sequences revealed 15 haplotypes of cytochrome oxidase subunit II (COII) gene in the world. Two haplotypesofCOIIwereidentifiedinMississippi.Inaddition,specificprimersweredesignedandtesteddifferentiallytoamplify characteristic fragments for verifying and surveying different genotypes ofC.formosanusin the future. CONCLUSION: Of the two haplotypes identified in Mississippi, the GA group was identical to those reported previously in Georgia, Louisiana, Alabama and other infested states. The second haplotype, the AT group, was identified for the first time in southeastern United States. Sequence identity of the AT-group C. formosanus with those reported mainly in southeastern Asian countries provided evidence of at least two introductions of C.formosanusinto the United States. c � 2010 Society of Chemical Industry |
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