Molecular genetic analysis of DNA polymerase and thymidine kinase genes of a HSV-1 population using an MPS technology

Autor:	A. A. Gus’kova, Alexander Lavrov, M. Yu. Skoblov, G. A. Galegov, D. V. Goldshtein, V. L. Andronova, Yu. S. Skoblov, D. A. Zubtsov
Rok vydání:	2013
Předmět:	education.field_of_study Massive parallel sequencing biology Hepatitis B virus DNA polymerase DNA polymerase viruses DNA polymerase II Population Biophysics Virology Molecular biology chemistry.chemical_compound chemistry Structural Biology Thymidine kinase biology.protein education Gene DNA
Zdroj:	Molecular Biology. 47:524-529
ISSN:	1608-3245 0026-8933
DOI:	10.1134/s0026893313040067
Popis:	Real-time PCR was used to determine the ratio of viral and host DNA in lysates of Vero cells infected with HSV-1 strain L2. The number of virus copies reached a maximum after 24 h of incubation. Total isolated DNA was sequenced using the massively parallel sequencing technique on an Ion Torrent apparatus. Nucleotide sequences of thymidine kinase (UL23) and DNA polymerase (UL30) genes of a HSV-1 L2 population were determined; their primary structures were compared to those of other standard HSV-1 strains, KOS and 17. The detected differences between the UL23 and UL30 sequences of L2 and reference strains KOS and 17 were unimportant because these substitutions did not affect the conserved gene regions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3dc4665483825fccdebd2718c304d8bd https://doi.org/10.1134/s0026893313040067 Zobrazit plný text záznamu Full text from SpringerLink