Insight into the Characteristics and New Mechanism of Nicosulfuron Biodegradation by a Pseudomonas sp. LAM1902
| Autor: | Qinghua Zhang, Rebecca E. Parales, Zhiyong Ruan, Qingyun Ma, Miaomiao Li, Xu Jiang, Delong Kong, Yiqing Zhou, Jinlong Song |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Arginine biology medicine.drug_class Chemistry 010401 analytical chemistry Pseudomonas Glyoxylate cycle General Chemistry Metabolism Biodegradation biology.organism_classification 01 natural sciences Sulfonylurea Trehalose 0104 chemical sciences chemistry.chemical_compound Biochemistry medicine Proline General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 68:826-837 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.9b06897 |
| Popis: | A total of five strains of nicosulfuron-degrading bacteria were isolated from a continuously cultivated microbial consortium using culturomics. Among them, a novel Pseudomonas strain, LAM1902, with the highest degradation efficiency was investigated in detail. The characteristics of nicosulfuron-degradation by LAM1902 were investigated and optimized by response surface analysis. Furthermore, non-targeted metabolomic analysis of extracellular and intracellular biodegradation of nicosulfuron by LAM1902 was carried out by liquid chromatography/mass spectroscopy (LC-MS) and gas chromatography-time-of-flight/mass spectroscopy (GC-TOF/MS). It was found that nicosulfuron was degraded by LAM1902 mainly via breaking the sulfonylurea bridge, and this degradation might be attributed to oxalate accumulation. The results of GC-TOF/MS also showed that the intracellular degradation of nicosulfuron did not occur. However, nicosulfuron exerted a significant influence on the metabolism of inositol phosphate, pyrimidine, arginine/proline, glyoxylate, and dicarboxylate metabolism and streptomycin biosynthesis. The changes of myo-inositol, trehalose, and 3-aminoisobutanoic acid were proposed as a mechanism of self-protection against nicosulfuron stress. |
| Databáze: | OpenAIRE |
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