Physiological features of rapeseed plants expressing the gene for an antimicrobial peptide cecropin P1

Autor:	Ya. I. Buryanov, M. A. Chepurnova, S. V. Pigoleva, N. S. Zakharchenko, E. V. Loktyushov, Vladimir D. Kreslavski, D. V. Vetoshkina, A. A. Kosobryukhov, A. A. Lebedeva
Rok vydání:	2013
Předmět:	biology Agrobacterium Transgene fungi Brassica food and beverages Plant Science Genetically modified crops Biotic stress Erwinia biology.organism_classification Antimicrobial Fusarium sporotrichioides Microbiology
Zdroj:	Russian Journal of Plant Physiology. 60:411-419
ISSN:	1608-3407 1021-4437
DOI:	10.1134/s1021443713030163
Popis:	Transgenic rapeseed (Brassica napus L.) plants carrying an artificial gene for the antimicrobial peptide cecropin P1 (cecP1) were obtained and characterized. The agrobacterial transformation was done by vacuum infiltration of seeds with agrobacterium GV3101(pMP90RK) containing a binary vector pGA482::cecP1. The cec1 gene expression was analyzed by Western blotting and confirmed by antimicrobial activity measurements of plant extracts. The obtained plants showed the resistance to the bacterial and fungal pathogens Erwinia carotovora and Fusarium sporotrichioides. The photosynthetic activities of control and transgenic plants under biotic stress conditions of E. carotovora infection were comparatively studied. The higher tolerance of the cecP1 plants to the oxidative stress caused by paraquat was shown. The results obtained point to the possibility of incorporation of the cecropin P1 gene into the integral stress protection system of plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3db2582382700ec0b009e8876ebc9f4f https://doi.org/10.1134/s1021443713030163 Zobrazit plný text záznamu Plný text ve formátu PDF