Quantitative Determination of Anastrozole by Liquid Chromatography–Tandem Mass Spectrometry
| Autor: | I. I. Miroshnichenko, N. I. Yurchenko, A. I. Platova |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Chemical ionization Column chromatography Chromatography Liquid chromatography–mass spectrometry Chemistry Drug Discovery Selected reaction monitoring Solid phase extraction Direct electron ionization liquid chromatography–mass spectrometry interface Mass spectrometry Triple quadrupole mass spectrometer |
| Zdroj: | Pharmaceutical Chemistry Journal. 47:231-234 |
| ISSN: | 1573-9031 0091-150X |
| Popis: | Anastrozole is a potent and selective non-steroidal aromatase inhibitor and is used for therapy of postmenopausal women with hormone-receptor-positive breast cancer. This paper provides a detailed description of the determination of anastrozole in human plasma by liquid chromatography–mass spectrometry (LC–MS–MS). Plasma-sample pretreatment involved solid-phase extraction using Accubond SPE ODS-C18 cartridges. Chromatographic separation was carried out on a Zorbax Bonus-RP column with a mobile phase consisting of aqueous formic acid (0.2%) (eluent A) and MeOH (eluent B) with a ratio A:B = 30:70 (v/v) that was supplied at flow rate 0.2 mL/min. Detection was performed on an Agilent 6410-2K triple quadrupole tandem mass spectrometer by multiple reaction monitoring in the preset mass range 294.2 → 225.1 using atmospheric-pressure chemical ionization in positive-ion mode. A calibration curve for anastrozole was linear (R2 ≥ 0.97) over the concentration range 0.5 – 20.0 ng/mL with a lower detection limit of 0.5 ng/mL. The described procedures can be applied successfully to both therapeutic monitoring of anastrozole and drug bioequivalence studies. |
| Databáze: | OpenAIRE |
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