Effects of Vitamin D Metabolites on Mineralization of Embryomic Chick Femurs In Vitro

Autor:	Shigeshi Kikunaga, Tadahiro Kikukawa
Rok vydání:	1997
Předmět:	Pharmacology medicine.medical_specialty biology Acid phosphatase Organ culture Mineralization (biology) Bone resorption In vitro Endocrinology Vitamin D+Metabolites Internal medicine medicine biology.protein Alkaline phosphatase Increased bone formation
Zdroj:	Proceedings of The Japanese Society of Animal Models for Human Diseases. 13:53-57
ISSN:	1884-4197 0918-8991
DOI:	10.1538/expanim1992.13.53
Popis:	We examined the effects of vitamin D metabolites on mineralization of the 10-day embryonic chick femurs in organ culture system. When 5-day cultures kept in the control medium were cultured in the several mediums containing 1α (OH) D3, 1, 25 (OH) 2D3 or 24, 25 (OH) 2D3 at concentration of 10-11-10-8 M during an additional 5 days, the mineralization of cultured bones increased in the treatment with 1α (OH) D3 at concentration of 10-10-10-8M, and decreased in the treatment with 1, 25 (OH) 2D3 at concentration of 10-10-10-8 M, and was no change in the treatment with 24, 25 (OH) 2D3. The activities of both alkaline phosphatase and acid phosphatase did not change in the cultured bones with vitamin D metabolites. These results suggested that 1α (OH) D3 increased bone formation, 1, 25 (OH) 2D3 stimulated bone resorption, and 24, 25 (OH) 2D3 had no effect to bone-remodeling in this organ culture system.
Databáze:	OpenAIRE
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