Cell migration and organization in three-dimensional in vitro culture driven by stiffness gradient
| Autor: | K. Jimmy Hsia, Ann M. Nardulli, Yutao Han, Christopher Blasius, Jing Jiang, Daniel Perlitz, Gubeum Kwon, Yvonne S. Ziegler, Michael Grigola, Danielle N. Joaquin |
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| Rok vydání: | 2016 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine animal structures Materials science Bioengineering Nanotechnology macromolecular substances 02 engineering and technology Applied Microbiology and Biotechnology Extracellular matrix 03 medical and health sciences medicine Substrate stiffness Cell mechanics Durotaxis technology industry and agriculture Stiffness Cell migration Cell movement equipment and supplies 021001 nanoscience & nanotechnology Finite element method 030104 developmental biology medicine.symptom 0210 nano-technology Biological system Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 113:2496-2506 |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.26010 |
| Popis: | Durotaxis, a phenomenon that cells move according to changes in stiffness of the extra cellular matrix, has emerged as a crucial parameter controlling cell migration behavior. The current study provides a simple method to generate three-dimensional continuous stiffness variations without changing other physical characteristics of the extra cellular environment. Using Finite Element simulations, the stiffness and the stiffness gradient variations are evaluated quantitatively, leading to an analysis of the dependence of cell migration behavior on the substrate stiffness parameters. We tested various cell lines on several 3-D environments. The durotaxis results show that the cell migration velocity does not have any consistency with the stiffness of the substrate, rather it is more related to the stiffness gradient of the substrate. This finding suggests a new mechanism underlying the durotaxis phenomenon, highlighting the importance of the substrate stiffness gradient, rather than the stiffness itself. Biotechnol. Bioeng. 2016;113: 2496-2506. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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