| Autor: |
Bram Timmerarends, C. D. de Gooijer, Peter Skrabal, Elmar Heinzle, Georg Schmid, Johannes Tramper, Ahmet Ozemre, H.P.J. Bonarius |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Animal Cell Technology ISBN: 9789401062732 |
| DOI: |
10.1007/978-94-011-5404-8_99 |
| Popis: |
The analysis of the intracellular metabolic fluxes in mammalian cells can be used for optimizing culture media, for tracing metabolic bottle-necks and for toxicological and metabolic studies. It is desirable to determine metabolic fluxes without using isotopic tracers. This could potentially be achieved by using the material balances of the relevant metabolites. For each metabolite a mass balance is set up using the known biochemical stoichiometry and its measured uptake or production rate. Together, these mass balances form a linear set of equations, containing the unknown fluxes. However, such a set of linear equations is underdeterwined due to the presence of cyclic pathways in mammalian cell metabolism. Fluxes in such pathways have to be quantitated using isotopic tracers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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