A RAPID METHOD FOR ESTIMATING VIABILITY IN DESICCATED CELLS OF THE BIOCONTROL AGENTTSUKAMURELLA PAUROMETABOLAC-924

Autor:	Neylen Del Toro, Jesús Mena, Armando Hernández, Alain Moreira, Jorge Martínez, Eulogio Pimentel
Rok vydání:	2008
Předmět:	Absorbance Chromatography Strain (chemistry) Significant difference Tsukamurella paurometabola Common logarithm Biology Microbiology
Zdroj:	Journal of Rapid Methods & Automation in Microbiology. 16:222-229
ISSN:	1745-4581 1060-3999
DOI:	10.1111/j.1745-4581.2008.00129.x
Popis:	A new method for the rapid estimation of viability in anhydrobiotic cells of the biocontrol agent Tsukamurella paurometabola C-924 was developed. The method is based on the absorbance due to hydrogen sulfide production. A linear model was obtained by plotting decimal logarithm of the corrected absorbance at 670 nm (log(Abscorrected)) versus the decimal logarithm of cul- turable cells (log(Xv)) (r 2 = 0.9428). After comparing the results obtained by the proposed method to the ones obtained by the plate counting technique, no significant difference was observed in the number of culturable cells. The new technique, which is faster than plate counting, permits the estimation of biologic activity in anhydrobiotic cells of the strain C-924 in only 2 h.
Databáze:	OpenAIRE
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