Fluorimetric and colorimetric detection of nitric oxide in living cells by rhodamine derivatives assisted by Cu2+

Autor:	Yixin Zhang, Dong-Yan Hou, Chunying Duan, Guang Xin, Cheng He, Tiechun Li, Qingtao Meng
Rok vydání:	2013
Předmět:	Rhodamine Detection limit Hepes buffer chemistry.chemical_compound Fluorescence-lifetime imaging microscopy chemistry Organic Chemistry Drug Discovery Inorganic chemistry Biochemistry Intracellular Nitric oxide
Zdroj:	Tetrahedron. 69:636-641
ISSN:	0040-4020
DOI:	10.1016/j.tet.2012.11.010
Popis:	Two rhodamine-based NO probes were designed and synthesized. Both RB-TP and RB-Py can detect NO by spectroscopic and ‘naked-eye’ methods in HEPES buffer assisted by Cu2+. The detection limits for NO were 5 nM and 20 nM, respectively. Other biologically relevant reactive species, such as NO2−,NO3−,OONO−, H2O2, 1O2, and ClO− did not give any obvious interferences. RB-TP and RB-Py have been also applied to detect the intracellular NO by fluorescence imaging method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3d38310a0be28e84c215a0c089a4908b https://doi.org/10.1016/j.tet.2012.11.010 Zobrazit plný text záznamu Full Text from ScienceDirect