A Gas Chromatographic Procedure for Determining Soluble Carbohydrates Extracted from Leaf Tissue of Ilex opaca Ait. Cv. ‘Miss Helen’1
| Autor: | T. A. Fretz, C. W. Dunham, C. W. Woodmansee |
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| Rok vydání: | 1970 |
| Předmět: | |
| Zdroj: | Journal of the American Society for Horticultural Science. 95:99-102 |
| ISSN: | 2327-9788 0003-1062 |
| DOI: | 10.21273/jashs.95.1.99 |
| Popis: | Leaf tissue carbohydrates of Ilex opaca cv. ‘Miss Helen’ extracted with alcohol and methylated with trimethylchlorosilane (TMCS) and hexamethyl-disilizane (HMDS) were assayed by gas chromatography. This empirical procedure involved a one-hour refluxing of 1 g of air dried plant tissue in 80% ethanol, followed by taking 2 ml of the alcoholic extract to dryness at 70°C. Upon dryness the material was taken up with 0.25 ml anhydrous pyridine, and methylated with 0.1 ml of TMCS and 0.1 ml of HMDS. After 30 min of reaction time a 2 µl portion of the mixture was assayed by gas chromatography. Five carbohydrates, D-fructose, D-galactose, alpha-D-glucose, beta-D-glucose and sucrose were identified. These carbohydrates extracted from the plant tissue were of a soluble nature. No apparent starch or cellulose hydrolysis was observed as a result of the extraction procedure. Reagent grade carbohydrates were observed to anomerize during the extraction procedure, however no indication of this phenomenon was noted with the plant tissue. Recovery was in the order of 90% with good associated reproducibility. |
| Databáze: | OpenAIRE |
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