Protection of Polynucleotide Phosphorylase by Substrates against Urea Inactivation

Autor:	J. Lucas-Lenard, M. Grunbebg-Manago, R. A. Harvey, T. Godefroy
Rok vydání:	1967
Předmět:	chemistry.chemical_classification Ribonucleotide Chemistry Oligonucleotide Stereochemistry Polymer medicine.disease_cause Biochemistry chemistry.chemical_compound Polymerization Urea medicine Polynucleotide phosphorylase Escherichia coli Nucleoside
Zdroj:	European Journal of Biochemistry. 1:327-333
ISSN:	1432-1033 0014-2956
DOI:	10.1111/j.1432-1033.1967.tb00077.x
Popis:	Polynucleotide phosphorylase from Escherichia coli is irreversibly inactivated by preincubation with urea at concentrations of 3.0 M or greater. The addition of ribonucleotide polymers, oligonucleotides or nucleoside diphosphates to the preincubation medium markedly decreases the rate of this inactivation. Both oligonucleotides terminated with an unesterified 3′-hydroxyl group or esterified with a 3′-phosphate are equally effective.
Databáze:	OpenAIRE
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