A single-cell atlas of the developing Drosophila ovary identifies follicle stem cell progenitors
| Autor: | Maija Slaidina, Ruth Lehmann, Torsten U. Banisch, Selena Gupta |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Cell type Somatic cell Cell RNA Organogenesis Biology Germline Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis Genetics medicine Stem cell Progenitor cell 030304 developmental biology Developmental Biology |
| Zdroj: | Genes & Development. 34:239-249 |
| ISSN: | 1549-5477 0890-9369 |
| Popis: | Addressing the complexity of organogenesis at a system-wide level requires a complete understanding of adult cell types, their origin, and precursor relationships. The Drosophila ovary has been a model to study how coordinated stem cell units, germline, and somatic follicle stem cells maintain and renew an organ. However, lack of cell type-specific tools have limited our ability to study the origin of individual cell types and stem cell units. Here, we used a single-cell RNA sequencing approach to uncover all known cell types of the developing ovary, reveal transcriptional signatures, and identify cell type-specific markers for lineage tracing. Our study identifies a novel cell type corresponding to the elusive follicle stem cell precursors and predicts subtypes of known cell types. Altogether, we reveal a previously unanticipated complexity of the developing ovary and provide a comprehensive resource for the systematic analysis of ovary morphogenesis. |
| Databáze: | OpenAIRE |
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