Chromatographic behavior of anthocyanins on a C10CN stationary phase
| Autor: | Ya. Yu. Kul’chenko, V.I. Deineka, L. A. Deineka |
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| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Journal of Analytical Chemistry. 72:1233-1238 |
| ISSN: | 1608-3199 1061-9348 |
| DOI: | 10.1134/s106193481712005x |
| Popis: | The selectivity of the separation of some anthocyanins on Diasphere-11-C10CN stationary phase (phase I) is compared with the traditional reversed Symmetry C18 phase (phase II). It is found that, in contrast to phase II, phase I is effective in the separation of isomeric pairs of anthocyanins of 6-hydroxycyanidin- 3-rutinoside and delphinidin-3-rutinoside, 6-hydroxypelargonidin-3-rutinoside and cyanidin-3-rutinoside, which ensures the determination of anthocyanins of Alstroemeria flowers. A comparison of separation maps shows that, on phase I, as compared with phase II, retention does not decrease so much, when OH groups are added to the anthocyanidin structure; trend lines for 3-mono-, di-, and triglucosides have a higher slope, and the addition of a glucosidic substituent at position 5 results in a more significant decrease in the retention of anthocyanins. Different selectivity of the separation of anthocyanins on phase I makes this separation version a good alternative to traditional reversed phase chromatography. |
| Databáze: | OpenAIRE |
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