Autor: |
Ľuboš Vrtoch, Martin Pipíška, Miroslav Horník, Jozef Augustín |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
Nova Biotechnologica et Chimica. 7:41-49 |
ISSN: |
1339-004X |
DOI: |
10.36547/nbc.1357 |
Popis: |
Tobacco has previously been used in investigations of metals and radionuclide uptake. This study presents determination of bioaccumulation and translocation of 60Co2+ ions in tobacco plants (Nicotiana tabacum L.) grown in Hoagland’s nutrient solution. Cobalt concentration in tobacco plants increased with increasing concentration in nutrient solution. Bioaccumulation from the initial concentration C0 = 0.96 μM Co reached 90% after 7 day cultivation. Only small amounts of Co accumulated in roots, up to 2 - 4 % were removable from roots by washing with 0.1 M CoCl2, indicating that this portion of Co is bound to the root surface in ion-exchangeable form. Tobacco roots retained approximately 2/3 of accumulated cobalt and 1/3 was transported to shoots. Autoradiography revealed that 60Co was preferentially localized in younger leaves. Prolongation of cultivation time did not change the [Co]roots : [Co]shoots ratio significantly. Relationships between growth rate, transpiration rate, uptake and distribution of cobalt in plant tissue are discussed. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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