| Popis: |
The mouse and rat are often the species of choice in standard toxicologj-cal testing. However, due to potential differences in chemical metabolism between these species and humans, they may in some instances be inappropriate models for chemical safety assessment. Because the guinea pig is a well-known model for chemical sensitization and hypersensitivity testing, the objective of the present study was to develop the hemolytic plaque assay in the guinea pig for use as an immunotoxicological endpoint in toxicology studies where metabolic differences between this and other rodent species may affect risk characterization. The present study encompassed six experiments designed to address route of administration, appropriate number of sheep red blood cells (sRBC) for immunization, time course after immunization, and a validation experiment to test the ability to detect suppression produced by a known immunosuppressant. Animals were observed daily for morbidity, mortality, and any signs of distress. Animals were... |
