CLONING OF ENTEROBACTER CLOACAE CEPHALOSPORINASE GENE AND ITS EXPRESSION IN ESCHERICHIA COLI

Autor:	Teizo Murata
Rok vydání:	1985
Předmět:	Cloning Genetics animal structures urogenital system Structural gene Biology biology.organism_classification medicine.disease_cause Plasmid embryonic structures medicine Hybrid plasmid Enterobacter cloacae Gene Escherichia coli
Zdroj:	The KITAKANTO Medical Journal. 35:205-212
ISSN:	1883-6135 0023-1908
DOI:	10.2974/kmj1951.35.205
Popis:	The chromosomal gene for cephalosporinase (CSase) production by Enterobacter cloacae GN7471 was cloned into vector plasmid pACYC 184. From the substrate profile and the response to anti-E. cloacae CSase serum, it was shown that the hybrid plasmid (pMS161) carries the chromosomal CSase gene of E. cloacae GN7471. E. cloacae CSase encoded by the cloned plasmid was produced in an inducible manner in both E. cloacae and E. coli, but its expressed specific activity was much lower than that of the original E. cloacae. From the analysis of several hybrid plasmids derived from pMS161, it was deduced that the site of the structural gene of CSase is located in a 1.15-Md fragment and the gene regulating the inducibility of CSase prodution in a 0.3 Md fragment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3cd839e4c997a016edd6bcffcb7a02af https://doi.org/10.2974/kmj1951.35.205 Zobrazit plný text záznamu