Agrobacterium-mediated transformation of lettuce (Lactuca sativa L.) with genes coding bacterial antigens from Mycobacterium tuberculosis

Autor:	N. V. Kuchuk, M. Yu. Vasylenko, A. M. Shakhovsky, N. A. Matvieieva
Rok vydání:	2009
Předmět:	Agrobacterium fungi food and beverages Lactuca Cell Biology Biology biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Molecular biology Genome chemistry.chemical_compound Plasmid chemistry Transcription (biology) Botany Genetics Bacterial antigen Gene DNA
Zdroj:	Cytology and Genetics. 43:94-98
ISSN:	1934-9440 0095-4527
Popis:	Transgenic lettuce plants (Lactuca sativa L.) with genes coding the synthesis of tuberculous antigens were obtained using the Agrobacterium-mediated transformation procedure. Cotyledonary leaves of in vitro lettuce seedlings (cvs. Eralash, Snezhinka, and Rubinovoe Kruzhevo) were transformed with plasmids containing nptII, a selective neomycin phosphotransferase II gene, and ESAT6, Ag85B (-TMD), and ESAT6:Ag85B (-TMD) target genes. A PCR analysis of the genome’s DNA confirmed the presence of both selective and target genes in all plants examined. At the same time, RT-PCR analysis showed that, in the case of stable transcription of the nptII gene, both the presence and absence of transcription of the ESAT6 gene are possible.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3cd4dc9744b42082ad44bca22559865b https://doi.org/10.3103/s0095452709020042 Zobrazit plný text záznamu Full text from SpringerLink