The Characterization of an Easy-to-Operate Inexpensive Student-Built Fluorimeter

Autor:	Rod Kreuter, Dan Sykes, Benjamin T. Wigton, Balwant S. Chohan
Rok vydání:	2011
Předmět:	Science instruction business.industry Fluorometer Silicon photodiode Nanotechnology General Chemistry business Computer hardware Education Characterization (materials science)
Zdroj:	Journal of Chemical Education. 88:1188-1193
ISSN:	1938-1328 0021-9584
DOI:	10.1021/ed100958z
Popis:	A compact, rugged, and portable fluorimeter was designed and constructed using a 460 nm light-emitting diode for the excitation and a silicon photodiode for detection. The total cost of such an instrument is less than $110, thus allowing for deployment of multiple instruments at low cost. The fluorimeter is easy to operate and has been successfully used to quantify fluorescein in commercial-brand antifreeze and chlorophyll a pigment in spinach leaves. The mini instrument and the associated experiments have provided for a novel approach to teaching the principles and applications of spectroscopy and fluorescence to a wide academic variety of students.
Databáze:	OpenAIRE
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