TNFα and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage
| Autor: | Jolana Lipková, Anna Vašků, Martin Smrčka, Zbyněk Šplíchal, Tereza Madaraszova, Kamil Ďuriš, Michal Jurajda |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Subarachnoid hemorrhage business.industry medicine.medical_treatment Inflammation medicine.disease Pathophysiology nervous system diseases Endocrinology Cytokine Apoptosis Internal medicine microRNA medicine Surgery Tumor necrosis factor alpha cardiovascular diseases Neurology (clinical) medicine.symptom business Stroke |
| Zdroj: | Česká a slovenská neurologie a neurochirurgie. :53-59 |
| ISSN: | 1802-4041 1210-7859 |
| DOI: | 10.14735/amcsnn201953 |
| Popis: | Aims: The aim of the present study was to investigate expression changes of pro-inflammatory and pro-apoptototic cytokine TNFα (Tumor Necrosis Factor-alpha) and microRNAs involved its regulation in early pathophysiological changes after SAH. Materials and Methodology: MicroRNA (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNFα) expression were determined by quantitative real-time PCR in brain tissue samples. A total of 88 animals were divided to Sham, Mild SAH, Severe SAH groups in following time-points: 2h, 4h, 6h, 8h (n=7per group) including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNFα expression between Sham and Severe SAH groups at all the time-points (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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