Optimization of the cultural medium and conditions for production of antifungal substances by Streptomyces platensis 3-10 and evaluation of its efficacy in suppression of clubroot disease ( Plasmodiophora brassicae ) of oilseed rape
| Autor: | Weidong Chen, Long Yang, Jing Zhang, Shouwen Chen, Ang Lyu, Qaiser Shakeel, Mingde Wu, Guoqing Li |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Starch 030106 microbiology Aspergillus niger Soybean meal Biological pest control biology.organism_classification medicine.disease 01 natural sciences Spore Clubroot 03 medical and health sciences chemistry.chemical_compound chemistry Insect Science Botany medicine Yeast extract Food science Agronomy and Crop Science Incubation 010606 plant biology & botany |
| Zdroj: | Biological Control. 101:59-68 |
| ISSN: | 1049-9644 |
| DOI: | 10.1016/j.biocontrol.2016.06.007 |
| Popis: | This study was conducted to optimize the cultural medium and conditions for production of antifungal substances by Streptomyces platensis 3-10 and to evaluate its efficacy in suppressing Plasmodiophora brassicae (PB), the causal agent for clubroot of oilseed rape. The optimization was done using the one-factor-at-a-time (OFAT) method, followed by orthogonal designing. The optimized medium contained starch at 3% (w/v), peptone at 0.75%, yeast extract at 0.025%, soybean meal at 1% (w/v) and minor/trace elements (K+, Mg2+, Mn2+, Zn2+), initial pH 6.5. After incubation in this medium at 28 °C/150 rpm for 72 h, isolate 3-10 showed high antifungal activity. The cultural filtrates (CF3-10) produced an average inhibition-zone diameter of 50 mm against Aspergillus niger. The live spores and metabolites of 3-10 in CF3-10 or its crude extracts (CE3-10) suppressed PB infection of oilseed rape roots by 3–83%, compared to the treatment with PB alone. The efficacy was positively correlated (correlation co-efficiency: 0.9526–0.9873, P |
| Databáze: | OpenAIRE |
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