Augmenting salt tolerance in rice by regulating uptake and tissue specific accumulation of Na + ‐ through Ca 2+ ‐induced alteration of biochemical events

Autor: Amber Gupta, Birendra Prasad Shaw
Rok vydání: 2021
Předmět:
Zdroj: Plant Biology. 23:122-130
ISSN: 1438-8677
1435-8603
Popis: Protective effect of Ca2+ against NaCl toxicity was investigated in two rice varieties contrast for salt tolerance to understand the mechanistic details of the antagonism so as to address the issue of an adverse effect of salinity on agriculture. The study primarily included understanding the influence of Ca2+ on the expression/activity of the effectors and regulators involved in Na+ translocation. Ca2+ led to reduced uptake of Na+ concomitant with a higher tissue K+ /Na+ ratio in the seedlings, comparatively more in the salt-tolerant Nona Bokra than in the -sensitive IR-64 together with significant increase in the root PM-H+ ATPase in the former, but not in the latter. A greater antagonism observed in Nona Bokra could be a result of Ca2+ signaling-mediated phosphorylation of PM-H+ ATPase in its root caused by a significant Ca2+ -dependent increase in expression of OsCIPK24, which did not happen in IR-64. Furthermore, significant Ca2+ -mediated NaCl-induced increase in transcription of 14-3-3 protein in Nona Bokra, but not in IR-64 could also be the reason of a greater protective effect of Ca2+ in the former than in the latter, as 14-3-3 protein is essential for activating PM-H+ ATPase. Thus, an efficient functioning of PM-H+ ATPase could be the key factor determining resistance of plants to salinity, implying that identification of the Ca2+ -dependent kinase phosphorylating the PM-H+ ATPase threonine residue and manipulation of its expression together with the expression of 14-3-3 proteins could be an important strategy in improving salt tolerance of the crop cultivars of interest for their cultivation in salt-affected lands.
Databáze: OpenAIRE
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