ASSESSMENT OF GENETIC VARIATION IN CITRUS GERMPLASM OF SOUTH OF IRAN USING SSR MARKERS

Autor:	Kourosh Vahdati, M. Danesh, B. Golein, Alireza Nabipour, A. Khadem Nematollahi
Rok vydání:	2009
Předmět:	Germplasm biology food and beverages Horticulture biology.organism_classification chemistry.chemical_compound Geography Rutaceae chemistry Genetic marker Molecular marker Genetic variation Genotype Botany Microsatellite Genetic variability
Zdroj:	Acta Horticulturae. :549-554
ISSN:	2406-6168 0567-7572
Popis:	In this study, eight SSR markers were used to assess the genetic variation among 48 citrus genotypes (12 orange, 6 grapefruit, 20 mandarin and 10 lemon) from the Citrus repository of the Agricultural Research Institute of South of Iran. From each accession, a full-grown tree was selected and genomic DNA was extracted from young leaves using a CTAB procedure. DNA amplification was performed and SSR banding patterns were evaluated in silver-stained polyacrylamide gels. The allele bands were transformed into binary data of presence-absence and resulting matrices were processed by NTSYS software. In total, 32 alleles were detected using the eight SSR primer pairs. The number of putative alleles per primer pair ranged from three to seven with an average of four. Polymorphic Information Content (PIC) values varied from 0.31 to 0.82. Based on the marker data, cluster analysis separated the Citrus collections into two groups. This study detected genetic similarities and differences between citrus germplasm of south of Iran.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3c9be76f59960a4685a5f1c29b085094 https://doi.org/10.17660/actahortic.2009.839.75 Zobrazit plný text záznamu