MICROPROPAGATION, DETECTION AND ELIMINATION OF DMV IN THE CZECH COLLECTION OF DAHLIA

Autor:	J. Sediva, J. Laxa, J. Kanka, P. Novak
Rok vydání:	2006
Předmět:	Dahlia biology ved/biology fungi ved/biology.organism_classification_rank.species food and beverages Horticulture Meristem biology.organism_classification Dahlia pinnata chemistry.chemical_compound Tissue culture Murashige and Skoog medium chemistry Micropropagation Botany Zeatin Explant culture
Zdroj:	Acta Horticulturae. :495-498
ISSN:	2406-6168 0567-7572
DOI:	10.17660/actahortic.2006.725.71
Popis:	Production of virus free dahlia plants is necessary for the maintenance of the Czech collection of Dahlia pinnata. The effects of cytokinins (BA and zeatin) and time of sampling of primary explants were investigated. Higher regeneration rate of primary explants was observed when explants were grown on MS medium with 0.5 mg L -1 zeatin. Dahlia mosaic virus (DMV) could be eradicated from infected plants by meristem-tip culture. The effects of size of meristem tips on virus elimination and meristem regeneration were investigated. Apical meristems excised from in vitro culture of Dahlia were cultivated on MS medium with zeatin. Efficiency of meristem culture in elimination of DMV was 50% and 9% for plantlets developed from meristems in size 0.4-0.5 mm and 0.9-1 mm, respectively. Real-time PCR (polymerase chain reaction) and conventional PCR assay were used for detection of DMV.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3c8efe19fd09a702b4bc4ca3dff7e9ba https://doi.org/10.17660/actahortic.2006.725.71 Zobrazit plný text záznamu