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Publisher Summary pBluescript vectors are multifunctional vectors. Many tests can be performed on the cloned insert with minimal effort and without requiring cumbersome subcloning steps into “specialized” vectors. Most of the features described in this chapter are available in kit format from commercial sources, which makes the experiments easier, more efficient, and reliable. pBluescriptII are phagemid vectors are multifunctional cloning vectors designed to simplify and expedite gene cloning and analysis. The vectors contain polylinkers that have been designed to optimize a number of cloning procedures, including directional cloning, gene mapping, unidirectional deletions of predictable length, T3 and T7 polymerase-mediated transcription, blue/white color selection for clones with insert, double-stranded DNA sequencing, and fl-galactosidase fusion protein expression. The fl filamentous phage origin of replication allows preparation of single-stranded DNA for mutagenesis and DNA sequencing. The ColE1 origin of replication and the ampicillin resistance gene allow propagation and selection of clones in plasmid form. |
