An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L
| Autor: | Ricardo Ernesto Bianchetti, Vinícius Sacramento Pacheco, Cristiano Ferrara de Resende, Aline Mystica Silva de Oliveira, Felipe Floriano Dornellas, J. C. E. Freitas, Paulo Henrique Pereira Peixoto |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology Inoculation Mimosa pudica biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Acclimatization In vitro 03 medical and health sciences 030104 developmental biology Murashige and Skoog medium Micropropagation chemistry Auxin Botany Genetics Agronomy and Crop Science Molecular Biology Ex vivo 010606 plant biology & botany Biotechnology |
| Zdroj: | African Journal of Biotechnology. 16:418-428 |
| ISSN: | 1684-5315 |
| DOI: | 10.5897/ajb2016.15831 |
| Popis: | This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies. Key words: Biodiversity conservation, ex vitro acclimatization, in vitro rooting, micropropagation, Mimosa pudica. |
| Databáze: | OpenAIRE |
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