Intracellular and extracellular sampling to monitor the neurotransmission process using a chromatographic method

Autor:	Lindsay D. Morgan, Bhavik Anil Patel, Leena Parmar
Rok vydání:	2011
Předmět:	Mechanism (biology) General Chemical Engineering General Engineering Sampling (statistics) Neurotransmission Biology Analytical Chemistry chemistry.chemical_compound Neurochemical Biochemistry chemistry Biophysics Extracellular Neurotransmitter Process (anatomy) Intracellular
Zdroj:	Analytical Methods. 3:2770
ISSN:	1759-9679 1759-9660
DOI:	10.1039/c1ay05520h
Popis:	The neurotransmission process is essential for cellular communication and in a variety of disorders the mechanism is altered. At present, biological tissue sampling approaches utilise single sampling techniques where either neurochemicals present inside the cell or released from the cell are monitored, yet the neurotransmission mechanism involves both processes. We have utilised a sampling approach that allows for monitoring intracellular and extracellular neurochemicals, using image analysis of the tissue area to normalise neurochemicals levels for both methods. Both ratios and neurochemical levels were presented to explain alterations in neurotransmission mechanism between proximal and distal colon. The new parameters obtained from intracellular and extracellular sampling are more suited to explain neurotransmitter release and clearance compared to conventionally used parameters from single sampling studies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3c667254aa5be25fa132a3f407d60fc0 https://doi.org/10.1039/c1ay05520h Zobrazit plný text záznamu